水稻白葉枯病菌生理小種在納米銀膜上的表面增強拉曼光譜初探

摘要：用硝酸銀和聚乙烯醇混合溶液作為電解液,把載玻片放入電解液中,用銀棒作為電極在直流電壓下電解1 h,使載玻片在電解出的銀膠中靜置12 h后,取小晾干,即得制備的銀膜.用便攜式拉曼光譜儀測得在銀膜上的白葉枯病菌7個生理小種的表而增強拉曼光譜(SERS).7個生理小種分別是1-YN1,2-YN7,3-YN11,4-GD4 14,5-SCYC6,6-HEN11,7-FWJ,7個生理小種的SERS譜在峰位及峰的相對強度上都有很多差別.這可用于生理小種的鑒別.因為銀膜上的納米銀顆粒是銀膠中銀顆粒的聚合體,這種聚合體大大縮小了納米銀之間的距離,從而產生了很強的電磁增強作用.電解法制備的銀膜價格低廉,增強效果好,普通實驗室便可以制備.此研究為快速、方便檢測白葉枯病菌不間生理小種提出了一種新的方法,對白葉枯病的防治具有重要的意義.Abstract：The nano silver film was prepared by electrolysis method using silver nitrate and polyvinyl alcohol(PVA)in deionized water as the electrolyte,with four glass slides put in the electrolyte and two silver rods dipped into the electrolyte as the anode and cathode.A direct current was applied to the rods,then the four glass slides stayed in the silver colloids.Thus the authors got the nano silver film.Transmission electron microscopy(TEM)and scanning electron microscopy(SEM)were employed to detect the silver particles in the silver colloids and on the nano silver film.From the SEM we can see that the silver particles on the film formed different layers.In one layer,the distance between two particles was about 100 nm.The samples of Xanthomonas oryzae pv.Oryzae(Xoo)were 7 different kinds of bacterial blight,namely 1-YN1,2-YN7,3-YN11,4-GD414,5SCYC6,6-HEN11 and 7-FWJ.Because the silver particles in the colloids were aggregated on the film,there was large electromagnetic potentiation.So the SERS spectra of Xoo were perfect.The authors used the area analytical method to distinguish the different kinds of Xoo.The silver film prepared by electrolysis was cheap and active,the preparation time of the samples was short,and any normal chemistry lab can make it,which can find excellent application to detecting the Xoo in agriculture.On the other hand,this film is active on biomolecules and bioorganisrn,which may be a new kind of SERS fundus to explain the creation of the SERS.Further study was under way. 作者： 康頤璞[1]司民真[1]李清玉[2]黃瓊[3]劉仁明[1] Author： KANG Yi-pu[1]  SI Min-zhen[1]  LI Qing-yu[2]  HUANG Qiong[3]  LIU Ren-ming[1] 作者單位： 楚雄師范學院物理與電子科學系,云南,楚雄,675000西南林學院基礎部,云南,昆明,650224云南農業大學植保學院,云南,昆明,650201 期 刊： 光譜學與光譜分析   ISTICEISCIPKU Journal： SPECTROSCOPY AND SPECTRAL ANALYSIS 年，卷(期)： 2010, 30(2) 分類號： Q331 關鍵詞： 白葉枯細菌    納米銀膜    電解法    SERS    Keywords： Xoo    Nano silver film    Electrolysis method    SERS    機標分類號： S51 S43 機標關鍵詞： 水稻    白葉枯病菌    病菌生理小種    納米銀顆粒    銀膜    表面增強拉曼光譜    Xanthomonas Oryzae pv.Oryzae    Spectra    SERS    silver film    scanning electron microscopy    Xanthomonas oryzae pv.Oryzae    different    電解液    polyvinyl alcohol    analytical method    bacterial blight    silver nitrate    direct current    電解法制備 基金項目： 國家自然科學基金，云南省基金

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