Determination of Trace Protein by Methylene Blue-Tetraphenylborate Fluorescence Probe Using Polyethylene Glycol as Sensi

An ionic association complex of methylene blue-tetraphenyiborate ([MB]+·[B(C6H5)4]-) can emit a strong and stable fluorescence in KH2PO4-Na2HPO4 buffer solution. In the presence of bovine serum albumin (BSA), the fluo-rescence signal of [MB]+·[B(C6H5)4]- can be sharply quenched, which can be further quenched by using polyeth-ylene glycol (PEG) as a sensitizer where the △F (△F=F0-F, F0 and F are the fluorescence intensities of the blank reagent and the test solution, respectively) of the system with PEG is 9.1 times higher than that without PEG, showing PEG has strong sensitizing effect on the quenching of a fluorescence signal. And there is a good linear correlation between △F and the content of BSA. Thus, a new fluorescence probe for the determination of trace pro-tein has been established, with the linear range of 0.11-88.0 pg·L-1 and the detection limit of 22.0 ag·mL-1 BSA. This sensitive method has been applied to the determination of protein in human serum samples with satisfactory results. And the reaction mechanism was also discussed. Under the same condition, the new method can be used to determie not only BSA, human serum albumin (HAS), ovalbumin (OVA) and γ-globulin (γ-G), respectively, but also the total protein in serum, brain and spinal cord.

作 者： LIU Jiaming LI Zhiming HUANG Xiaomei ZENG Liqing CHEN Xiaohua SONG Dongqin ZHENG Zhenzhen   作者單位： LIU Jiaming,ZENG Liqing,SONG Dongqin,ZHENG Zhenzhen(Department of Chemistry and Environmental Science,Zhangzhou Normal College,Zhangzhou,Fujian 363000,China)

LI Zhiming,CHEN Xiaohua(Department of Food and Biological Engineering,Zhangzhou Institute of Technology,Zhangzhou,Fujian 363000,China)

HUANG Xiaomei(Department of Chemistry and Environmental Science,Zhangzhou Normal College,Zhangzhou,Fujian 363000,China;Department of Food and Biological Engineering,Zhangzhou Institute of Technology,Zhangzhou,Fujian 363000,China) 

刊 名： 中國化學（英文版）  SCI 英文刊名： CHINESE JOURNAL OF CHEMISTRY  年，卷(期)： 2009 27(2)  分類號： O6  關鍵詞： protein   fluorescence quenching method   methylene blue-sodium tetraphenylborate fluorescence probe   polyoxyethylene   sensitizer  

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