- 相關推薦
Molecular Survey of Sulphate-Reducing Bacteria in the Deep-Sea Sediments of the West Pacific Warm Pool
The sulfate-reducing bacteria (SRB) community in the deep-sea sediments of the west Pacific Warm Pool (WP) was surveyed by molecular phylogenetic analyses using primers targeting the 16S rRNA gene fragments of SRB. Specific 16S rRNA gene libraries from five sediment layers (1-cm, 3-cm, 6-cm, 10-cm and 12-cm layer) of the 12-cm core of WP-0 were constructed. The clones in the five libraries were differentiated by restriction fragment length polymorphism (RFLP) and representative clones were selected to sequence. It was found that the clones fell into four groups, which were closest related to Desulfotomaculum, Desul- facinum, Desulfomonile and Desulfanuticus. Desulfacinum-like clones were only detected in the upper layers of the sediment core, whereas Desulfomonile-like clones were only present in the deeper layers. Fluorescence in situ hybridization (FISH) was further carried out to visualize and count the SRB and bacteria in the five sediment layers. It was found that SRB constituted only a small proportion of the bacteria community (0.34%-1.95%), it had the highest content in the 3-cm layer (1.95%) and had a depth- related decreasing tendency along the 12- cm core.
作 者： WANG Peng XIAO Xiang ZHANG Haiyan WANG Fengping 作者單位： WANG Peng(Laboratory of Marine Geology, Tongii University, Shanghai 200092, P. R. China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, P. R. China)XIAO Xiang,ZHANG Haiyan,WANG Fengping(Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, P. R. China)
刊 名： 中國海洋大學學報（英文版） ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA 年，卷(期)： 2008 7(3) 分類號： P7 關鍵詞： SRB west Pacific warm pool【Molecular Survey of Sulphate-Reducin】相關文章：
Molecular Evidences for the Biosynthesis of Pederin by Endosymbiont04-28
香港的樹木調測(TREE SURVEY)04-27
Alkyldibenzothiophenes:molecular tracers for filling pathway in oil reservoirs04-28
The molecular mechanism of embryonic stem cell pluripotency maintenance04-26
A SURVEY OF STACKELBERG DIFFERENTIAL GAME MODELS IN SUPPLY AND MARKETING CHANNELS04-30
Survey of polycyclic aromatic hydrocarbons (PAHs) in arterial street air of Hangzhou04-28
Molecular organic geochemistry of ornithogenic sediment from Svalbard, Arctic04-27
A SAPO-11 Silicoaluminophosphate Molecular Sieve with Stable Crystal Structure04-27