Cloning and expression of ophc2,a new organphosphorus hydrolase gene

The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63 %, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity.

作 者： WU Ningfeng DENG Minjie Liang Guoyi CHU Xiaoyu YAO Bin FAN Yunliu   作者單位： Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China  刊 名： 科學通報(英文版)  SCI 英文刊名： CHINESE SCIENCE BULLETIN  年，卷(期)： 2004 49(12)  分類號： Q7  關鍵詞： Pseudomonas pseudoalcaligenes   organphosphorus hydrolase   gene cloning and expression  

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